On the role of heparin and integrin binding segments in cell adhesion of biomaterials

Abstract

Event Abstract Back to Event On the role of heparin and integrin binding segments in cell adhesion of biomaterials Franziska Dreher1*, Mareen Pagel1* and Annette G. Beck-Sickinger1* 1 Universität Leipzig, Faculty of Biochemistry, Pharmacy and Psychology, Institute forBiochemistry, Germany Introduction: In order to improve functional biomaterials multifunctional coating is suggested. By using specific peptide sequences to mimic proteins of the extracellular matrix of the bone new approaches for improved implant integration have been suggested. Materials and Methods: The integrin binding sequence (c[RGDfK]) and a heparin binding sequence (FHRRIKA) were immobilized on titanium binding peptides. Cell adhesion, spreading, viability and proliferation of MG-63 cells on c[RGDfK] and FHRRIKA itself, in a combined peptide and in a 1:1 mixture were investigated in comparison to blank titanium, the titanium binding peptide itself and surface coating with fibronectin (FN). Results and Discussion: Only c[RGDfK] alone could markedly increase the number of adherent cells comparable to the level of FN, while cell spreading was elevated by all cell binding motifs. Hereby, c[RGDfK] and FHRRIKA itself and in a 1:1 mixture enhanced cell spreading to the same extend, while the combination of both motifs in one molecule increased cell size additionally. The coating combining c[RGDfK] and FHRRIKA also significantly increased cell viability compared to blank titanium. Viability was even higher than of FN, c[RGDfK] and FHRRIKA coating surfaces. Cell proliferation was generally enhanced on titanium compared to the tissue culture plate, but showed no significant differences with respect to the cell binding motif. Conclusion: These results demonstrate that short peptide sequences containing integrin and heparin binding motifs can efficiently enhance cell surface interactions on titanium and synergistically improve cell spreading and viability in MG-63 cells. TRR67/A4 Keywords: Bone Regeneration, Cell Adhesion, Biomimetic, RGD peptide Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Adhesive biomaterials Citation: Dreher F, Pagel M and Beck-Sickinger AG (2016). On the role of heparin and integrin binding segments in cell adhesion of biomaterials. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01960 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. * Correspondence: Dr. Franziska Dreher, Universität Leipzig, Faculty of Biochemistry, Pharmacy and Psychology, Institute forBiochemistry, Leipzig, Germany, Email1 Dr. Mareen Pagel, Universität Leipzig, Faculty of Biochemistry, Pharmacy and Psychology, Institute forBiochemistry, Leipzig, Germany, Email2 Dr. Annette G Beck-Sickinger, Universität Leipzig, Faculty of Biochemistry, Pharmacy and Psychology, Institute forBiochemistry, Leipzig, Germany, Email3 Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Franziska Dreher Mareen Pagel Annette G Beck-Sickinger Google Franziska Dreher Mareen Pagel Annette G Beck-Sickinger Google Scholar Franziska Dreher Mareen Pagel Annette G Beck-Sickinger PubMed Franziska Dreher Mareen Pagel Annette G Beck-Sickinger Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.