Abstract
The question is addressed, whether in capillary electrophoresis (CE) the laser-based forward degenerate four-wave mixing (F-D4WM) detection method can become a realistic alternative to conventional ultraviolet (UV) absorption detection. In order to answer this question F-D4WM experiments at 351 nm have been performed. As test analytes the herbicides dinitro- ortho-cresol and dinitro-( tert-butyl)- ortho-phenol (trivial names DNOC and dinoterb, respectively) were used without derivatisation. These compounds show moderate absorption (molar absorptivity 4000 M −1 cm −1) at 351 nm under the conditions (buffer pH 8.2) used for CE separation. The detection limits obtained (1 μM) were 25-fold better than with on-column absorption detection at 351 nm. This result is shown to be typical for CE, where commonly buffer solutions with a high water content are used. For micro-separation systems that are compatible with liquid solutions with a low water content F-D4WM detection has a much wider potential.
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