Abstract

Chloroacetaldehyde (CAA) and chloroethylene oxide (CEO), two reactive metabolites of vinyl chloride, were used to introduce increasing amounts of 1,N6-ethenoadenine (eA) and 3,N4-etheno cytosine (eC) residues in poly(dA) and poly(dC), respectively. The modified polynucleotides were assayed with E. coli DNA polymerase I for their template activity and for misincorporation. The miscoding properties of eA and eC that we observed may explain the mutagenic effects reported for vinyl chloride and its metabolites; these lesions may also represent one of the initial steps in vinyl chloride or CEO-induced carcinogenesis.

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