Abstract
The relative sedimentation rates of linear and covalently closed λ DNA in sucrose gradients containing varying amounts of tryptamine, tyramine, histamine and phenethylamine were measured. The presence of the aromatic amines does not significantly change the sedimentation coefficient of the closed DNA, indicating that the DNA helix rotation angle is not significantly altered. This lack of angular alteration is also supported by sedimentation measurements of λ DNA which had been covalently closed by ligase in the presence of tryptamine. These results suggest that intercalation of aromatic amino acid residues into DNA is unlikely, contrary to the conclusion drawn from other measurements.
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