On the possibilities of polychromatic synchrotron radiation microtomography for visualization of internal structures of Rhodnius prolixus

Abstract

The recent years advancements in microtomography have increased the achievable resolution and contrast, making this relatively inexpensive and a widely available technology, potentially useful for studies of insect’s internal morphology. Phase Contrast X-Ray Synchrotron Microtomography (SR-PhC-µCT) is a non-destructive technique that allows the microanatomical investigations of Rhodnius prolixus, one of the most important insect vectors of Trypanosoma cruzi, the etiologic agent of Chagas’ disease. In Latin America, vector control is the most useful method to prevent Chagas’ disease, and a detailed knowledge of R. prolixus’ interior structures is crucial for a better understanding of their function and evolution. Traditionally, in both biological morphology and anatomy, the internal structures of whole organisms or parts of them are accessed by dissecting or histological serial sectioning; so studying the internal structures of R. prolixus’ head using SR-PhC-µCT is of great importance in researches on vector control. In this work, volume-rendered SR-PhC-µCT images of the heads of selected R. prolixus were obtained using the new set-up available at the SYRMEP beamline of ELETTRA (Trieste, Italy). In this new set-up, the outcoming beam from the ring is restrained before the monochromator and in a devoted end-station, absorption and phase contrast radiography and tomography set-up are available. The images obtained with polychromatic X-ray beam in phase contrast regimen and 2 µm resolution, showed details and organs of R. prolixus never seen before with SR-PhC-µCT.