Abstract
t-RNA sequences have been aligned to maximize matches in corresponding positions. The sequences have subsequently been divided into two parts, the “squelette” (skeleton) and “muscle” positions. A test of homology based on the binomial approach has been developed and was applied to the “muscle” positions of t-RNA. The phylogenetic relationship and parts of ancestral sequences have been obtained from Val and Ser t-RNA families. The known Leu t-RNA sequences have been shown to be a part of two different homologous families, indicating the possibility that degenerate codons give rise to non-homologous isoaccepting t-RNA's.
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