Abstract
Frequency-resolved time correlated single photon counting experiments have been performed to investigate the time scales and molecular mechanisms for solvation dynamics of 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan) embedded in liquid-crystalline bilayers of unilamellar dimyristoyl-phosphatidylcholine (DMPC) vesicles. The reconstructed fluorescence spectra as a function of time exhibit a pronounced double-exponential Stokes shift, which is strongly correlated with the rotational diffusion dynamics of the chromophore as evidenced by experiments on transient fluorescence depolarization.
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