On the origin of ganoine: histological and ultrastructural data on the experimental regeneration of the scales of Calamoichthys calabaricus (Osteichthyes, Brachyopterygii, Polypteridae).

Abstract

In order to understand the process of ganoine formation on the ganoid scales, scale regeneration has been studied to overcome the lack of a growth series of scale ontogeny. Seven stages of ganoid scale regeneration have been defined over a period of five months in the polypterid fish Calamoichthys calabaricus. The study has been carried out using transmission electron microscopic techniques. After wound healing and differentiation of the osseous basal plate, a layer of vascular dentin is deposited at the upper surface of the basal plate owing to the presence there of odontoblasts closely applied to the dentin. When these cells move away, a close contact is then established between the stratified epidermis and the regenerating scale. Numerous alterations of the epidermal-dermal boundary occur until its disappearance and a thick layer of pre-ganoine is formed. This layer is progressively mineralized; and finally an organic intermediate layer differentiates between the ganoine, which is a hyper-mineralized tissue, and the overlying epidermis. This ultrastructural study demonstrates rather unequivocally the involvement of the inner epidermal layer (IEL) in the appearance and growth of the ganoine. It is suggested that these epidermal cells can be compared functionally to the inner dental epithelium (IDE) described during mammal tooth morphogenesis. Consequently, our results allow us to propose that ganoine can be identified as true enamel, although additional data are necessary to analyze the proteinaceous component or its organic matrix.