On the nature of pharyngeal muscle cells in the Tardigrada

Abstract

As part of a transmission electron microscopic study of the embryological development in tardigrades, the ultrastructure of the pharynx was examined. The intent was to establish whether the pharyngeal muscle cells constitute an eclodermal myoepithelium (as in many aschelminth pharynges) or whether they are mesodermal (as in certain other aschelminth groups and among articulates). In the latter case the cuticle would be produced solely by specialized epithelial cells. The eutardigrade species Halobiotus crispae Kristensen, 1982, was investigated in four embryological stages, as a newly hatched juvenile, in the active adult stage, and in the hibernation stage pseudosimplex I. A comparison was made with the arthrolardigrade Actinarctus doryphorus Schulz, 1935, in the active adult stage and in the simplex stage. The results indicate that the tardigrade pharynx is an ectodermal myoepithelium. The muscles appear to be truly cross-striated and monosarcomerial. The phylogenetic implications of these findings are discussed briefly.