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Abstract
1. Oxidation and phosphorylation coupled with endogenous substrate oxidation was studied in comparison with fatty-acid oxidation. Similar sensitivities to arsenite and malonate were observed for the oxidation of both endogenous substrate and fatty acid. The P:O ratio for the oxidation of endogenous substrate and fatty acids was found to be approx. 2.5. Endogenous substrate oxidation as well as fatty-acid oxidation was stimulated by NAD +. 2. The endogenous substrate was activated by both systems described by Van den Bergh for the activation of fatty acids. The carnitine-stimulated oxidation of endogenous substrate was observed only in the dinitrophenol-sensitive system. 3. The results presented indicate that fatty acids constitute the endogenous substrate in rat-liver mitochondria.
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