On the molecular identity of Paratylenchus nanus (Nematoda: Tylenchida).

Sergei A Subbotin,Mihail Kantor,Guiping Yan,Zafar Handoo

doi:10.21307/jofnem-2020-127

Abstract

In this study, molecular characterization of Paratylenchus nanus collected from the type locality in Four Mile Run, Fall Church, Virginia using COI, D2-D3 of 28 S rRNA and ITS rRNA gene sequences was provided. We molecularly also characterized, Paratylenchus specimens collected from grasses in Devils Lake, Ramsey County, North Dakota indicated as the type locality in the original description of P. nanus by Cobb (1923). These nematodes were identified as representatives of the species P. projectus. Populations of P. nanus belonging to the molecular types A and B, and previously designated by Van den Berg et al. (2014) should be now identified as P. nanus and P. projectus, respectively.

Highlights

Re-described and illustrated P. nanus from specimens collected from native sod near Devils Lake, North Dakota and designated the specimens as topotypes
This study showed that the populations identified as P. nanus might contain two sibling species, which were well separated using molecular
The objective of this work was to molecularly characterize P. nanus from the type locality, Four Mile Run, Fall Church, Virginia, USA designated by Tarjan (1960) and Paratylenchus species from the roots of grasses collected in Devils Lake, North Dakota, which was indicated as the type locality in the original description of P. nanus by Cobb (1923)

Summary

Introduction

Re-described and illustrated P. nanus from specimens collected from native sod near Devils Lake, North Dakota and designated the specimens as topotypes. Raski (1975) believed that the species from Devils Lake collected by Thorne and Smolik (1971) belonged to P. projectus Jenkins 1956, not to P. nanus, and designated new topotypes collected in 1958 from a grass soil sample in Fall Church, Virginia. Raski (1975) provided a detailed report of various collections and descriptions of the species He noticed that P. nanus was very similar to P. projectus. The objective of this work was to molecularly characterize P. nanus from the type locality, Four Mile Run, Fall Church, Virginia, USA designated by Tarjan (1960) and Paratylenchus species from the roots of grasses collected in Devils Lake, North Dakota, which was indicated as the type locality in the original description of P. nanus by Cobb (1923)

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