Abstract
The effect of acetate on the ability of renal cortical tissue to accumulate and to synthesize p-aminohippurate was determined in young and adult rats. Acetate increased the maximal rate of p-aminohippurate uptake in tissue from adult rats with no change in K m values. The effect of acetate on p-aminohippurate accumulation by renal cortical slices from adult rats was smaller than that from rabbits but the stimulatory effect was apparent. The p-aminohippurate slice/medium ( S M ) ratio was increased at high pH (8.2) and acetate increased further the p-aminohippurate S M ratio in both age groups. The ability of renal cortical homogenates to synthesize p-aminohippurate was used to estimate the activity of the enzyme glycine acyltransferase (EC 2.3.1.13). Enzyme activity was low in renal cortical homogenates from 10-day-old rats when measured under optimal conditions and with glycine rate limiting. Acetate did not change p-aminohippurate synthesis in tissues from young or adult rats. These data discount the hypothesis that acetate enhancement of p-aminohippurate transport is due to the formation of acetylglycine.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Biomembranes
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