On the limited peptic digestion of horse heart cytochrome C. isolation of C-terminal peptide sequences.

Abstract

Proteolysis of horse heart cytochrome C with pepsin for 3 min produces a large heme peptide, which was shown to be a mixture of the sequences 1–64 and 1–66. By ion exchange chromatography, gel filtration and preparative paper chromatography the heme‐free fraction was resolved into seven peptide fragments, corresponding to sequences 65–94, 65–96, 67–94, 67–96, 67–80, 95–104 and 97–104.