Abstract
The kinetics of inhibition by oligomycin of the mitochondrial ATPase and the Mg: ATP-dependent electrogenic transport of protons were compared in membrane preparations from radish seedlings. Oligomycin blocks the phosphohydrolyzing activity of mitochondrial ATPase (measured at pH 8.5) at 1 μg × mg −1 prot and the ATP-synthase one (measured at pH 6.6) at 3 μg × mg −1 prot. Oligomycin at these concentrations does not influence Mg: ATP-dependent proton transport and Mg: ATP-dependent hyperpolarization of transmembrane electric potential difference (PD) in microsomal vesicles. However, an inhibiting effect of the drug becomes apparent at concentrations higher than 10 μg × mg −1 prot, increasing with the increase of oligomycin concentration at least up to 240 μg × mg −1 prot. Both the vanadate and the NO 3 −-sensitive systems of Mg: ATP-dependent electrogenic transport of protons show similar sensitivity to oligomycin.
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