On the influence of protein aggregate sizes for the formation of solid and hollow protein microparticles

Abstract

Microparticles can function as carriers of e.g. pharmaceuticals and food ingredients. Hollow microparticles can enhance the capacitance due to their large interior void. For preparing microparticles, polymers have been assembled into spherical structures through the use of porous CaCO3 templates, followed by polymer cross-linking and selective template removal. However, this often results in the formation of microparticles with a solid core. Here we use proteins with different aggregate size distributions (<10 nm or >100 nm) to either form solid or hollow microparticles. Proteins were mixed with CaCl2 and Na2CO3 solutions, which from CaCO3 microcrystals (with 20–60 nm pores) with encapsulated proteins. Here it will be shown that small protein aggregates uniformly distributed into the CaCO3 templates. However, larger protein aggregates accumulated at the template edges. Au3+ ions were then added, which oxidize and cross-link proteins and are reduced to form gold nanoparticles (AuNPs). After removal of the templates, the small proteins formed solid microparticles and the larger protein aggregates hollow microparticles. This method of fabrication of solid and hollow protein microparticles, with embedded AuNPs, could be used for generating biomaterials with a broader range of applications, such as hosting molecules and multimodal imaging due to the presence of the AuNPs.