Abstract
The ligh polypeptide chains froms several purified anti-hapten rabbit antibodies and normal γ-globulin were prepared and subjected to acrylamide gel electrophoresis. The number and distribution of the bands formed were very similar in all cases. Antibodies to the 2,4 dinitrophenyl hapten, either pooled or from individual, allotypically homozygous, rabbits, were affinity labeled with tritiated m-nitrobenzenediazonium fluoborate. The labeling was confined to the antibody active sites, and was found on both heavy and light chains. The labeled light chains were subjected to gel electrophoresis and the label was found to be essentially uniformly distributed within the bands. These results are consistent with the suggestion that any population of anti-hapten antibodies contains a number of different kinds of light chains which is substantially larger than the number of electrophoretic bands, and by inference that the total number of possible light chains in a normal γ-globulin population may be very large indeed.
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