Abstract
Quantitative measurements of the different hemoglobin fractions obtained by CM-cellulose and Amberlite CG-50 chromatography and cellogel electrophoresis from normal and glucose-6-phosphate dehydrogenase-deficient adults are presented. The mean values, obtained by the three procedures, for the Hb-A 3 fraction of glucose-6-phosphate dehydrogenase-deucient red blood cells was lower than in normal red blood cells. (The difference was statistically significant in separations on CM-cellulose and cellogel electrophoresis). The mean values for the Hb-A 1 fraction, obtained by cellogel electrophoresis were significantly higher than m normal red blood cells. No significant difference in the amount of the Hb-A 2 fraction of the two kinds of red blood cells was observed. The significance of these results is discussed.
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