Abstract
The oxygen heterocyclic fraction of cold-pressed lemon essential oils has been studied by normal-phase HPLC. The components of the fraction have been isolated by column chromatography, TLC and semi-preparative HPLC with recycle. The identification of the isolated components has been carried out by 1H-NMR and mass spectrometry. Three coumarins (5-geranyloxy-7-methoxycoumarin, citropten, 5-isopentenyloxy-7-methoxycoumarin) and ten psoralens (bergamottin, 8-geranyloxypsoralen, oxypeucedanin, byakangelicol, oxypeucedanin hydrate, byakangelicin, imperatorin, phellopterin, isoimperatorin, 5-isopent-2′-enyloxy-8-(2′,3′-epoxyisopentyloxy)-psoralen) have been isolated and identified. The main components were bergamottin (160–291 mg/100 g of oil) and 5-geranyloxy-7-methoxycoumarin (180–250 mg/100 g of oil). Moreover, herniarin, a coumarin characteristic of lime essential oil, has been detected. Herniarin had been reported previously in essential oils obtained from Citrus limon L. var. Eureka. © 1998 John Wiley & Sons, Ltd.
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