On the existence of spontaneous neuronal bursting activity at the periphery of the amphibian auditory pathway

Abstract

The firing activity of 104 neurons from the medullar dorsal nucleus (homologous to the mammalian cochlear nucleus complex) of immobilized common frogs (Rana t. temporaria) was recorded extracellularly in the absence of external auditory stimuli. This background activity was analyzed by using stochastic point process parameters such as the coefficient of variation, local coefficient of variation, interdependence of neighboring intervals, interpulse interval distribution, autocorrelation, and hazard function. All these parameters were put in correspondence with bursting rate, i.e., relative number of interpulse intervals grouped in clusters, or bursts, determined by the proposed method. Neurons with pronounced refractoriness, as a rule, exhibited lower bursting rates whereas neurons with a distinct maximum of the correlation function showed higher values than expected from a random Poisson process. However, even in the latter group of cells, bursting rates usually did not decrease after random shuffling of all the interpulse intervals. As a result, we must admit the absence of clustered spontaneous activity in second-order auditory neurons in the amphibian species studied. These data are compared with the specificities of spontaneous firing activity in different parts of the mammalian auditory pathway.