Abstract
In an aqueous solution, fluorescein can exist in the form of several protolytic forms. Transitions between these forms in an excited state greatly complicate the process of studying the luminescent properties of individual components. For such a system, the emission spectrum depends on the excitation wavelength. In this paper, it is shown that for a sample having these properties, the universal Kennard thermodynamic relation for luminescence intensity is valid under any conditions, whereas the less general Kennard–Stepanov relation can be violated.
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