Abstract
A method outlined in a previous study (S.J. Gill, H.T. Gaud, J. Wyman and G. Barisas, Biophys. Chem. 8 (1978) 53) is applied for the determination of species fractions from ligand-binding data for the oxygen reaction with human hemoglobin. The results obtained by this alternative approach, which is based on the solution of a system of linear equations, are consistent with those obtained using nonlinear least-squares analysis.
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