Abstract
Since the ratio of total to eaterified cholesterol in blood may have diagnostic importance, particularly with reference to liver damage (l-6), the desirability of a rapid and accurate method for determining these substances is obvious. Most methods now in use involve the Liebermann-Burchard color reaction but, in many cases, no account is taken of the fact that esterified cholesterol produces more color in this reaction than does free cholesterol. Such procedures, of course, give results which are too high. This error is eliminated in the methods of Schoenheimer and Sperry (6) and of Kelsey (7) by the use of a saponification procedure. However, for routine use it would be desirable to eliminate this time consuming step. This study has shown that the color produced in the LiebermannBurchard reaction by the esterified cholesterol of calf blood is, within certain limits, a definite percentage greater than that produced by the same amount of free cholesterol. Hence, by application of an appropriate correction factor, it is possible to determine, with reasonable accuracy, total and esterified blood cholesterol without saponification.
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