Abstract
In order to detect the activated platelets, plasma beta-thromboglobulin (β-TG) levels and nucleotide pools in platelets were determined in blood samples from 34 diabetic patients with various vascular complications i. e. retinopathy, nephropathy, ischemic heart disease, cerebral thrombosis and obstruction of arteries in the extremities.β-TG levels were measured using RIA kit (Radiochemical Center, Amersham). Higher plasma, β-TG levels than 50ng/ml were found in seven patients (21%).Nucleotide pools in platelets were determined with high pressure liquid chromatography. ATP and ADP levels in platelets were decreased in 47% and 35 of the patients, respectively. ATP/ADP ratio in those cases was ranged between 0.9 and 2.8. Amount of GTP in platelets was elevated in 3 cases and decreasedd in 2 cases. All three cases whose plasma β-TG levels elevated more than 100ng/ml showed decrease of both ATP and ADP in the platelets.Measurement of nucleotide pools in platelets is a more sensitive method to detect the evidence of platelet activation in patients with arteriosclerosis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
