On the deposition of phospholipids onto planar supports with the Langmuir—Blodgett technique using factorial experimental design 1. Screening of various factors and supports

Abstract

Phosphatidic acid and a mixture of phosphatidic acid, phosphatidylcholine and cholesterol were transferred as an ordered monolayer to solid, hydrophilic supports by Langmuir—Blodgett technique. The supports were glass, chromium and platinum. Variables which might be of importance for obtaining good adhesion of the lipids to the support were studied. The variables, connected to lipid and lipid structure, subphase, transfer process and handling of the support, were investigated systematically, using fractional factorial design. No experimental condition was identified which would result in a good adhesion to glass supports. For chromium-coated and platinum-coated substrates, we found some variables having significant effects on adhesion. The results suggest that adhesion is correlated to the hydration of the support and lipids.