Abstract
The method devised by Boeck and Drbohlav for the cultivation ofE. histolyticahas been successfully used for cultivating this species and various other entozoic amoebae of man and monkeys.Various modifications of the original technique have been tried, and several improvements are here described. Among these are methods of cultivation (involving the addition of solid rice-starch to the medium) whereby (1) more luxuriant and prolonged growth of the amoebae, and (2) all stages—including encystation and excystation—in the life-cycle ofE. histolyticaand other species can be obtainedin vitro.Methods by which cultures of entozoic amoebae can be initiated from encysted forms have been discovered and are also described, and some indications are given for the isolation of pure strains from mixed cultures.The influence of the accompanying bacterial flora upon the various amoebae cultivated has been studied, and is briefly discussed in the light of certain experimental findings.Loss of infectivity to kittens has been observed inE. histolyticaas an apparent result of cultivation in media containing solid starch.
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