Abstract
Two types of mutants were used to study the control of transcription in phage P2. First, some amber mutants with strong polar effects were characterized. The properties of these mutants suggest that the late genes of P2 are arranged into at least four units of transcription. For three of these units, the polarity goes from left to right on the P2 map. Second, two independent mutations that make P2 insensitive to immunity have been located on the genetic map. Both mutations are located between the repressor gene C and the two early genes B and A. These mutations may affect the operator of an operon comprising genes B and A. The regulation of gene activity in P2 is discussed.
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