Abstract
1. Twenty ejaculates were collected from the same boar at weekly intervals; the composition of semen was studied by microscopic and chemical methods. The total volume of ejaculates varied from 240 to 500 ml., and sperm concentration from 50,000 to 177,000 cells/μl. Chemical analysis revealed 2·4–12·6 mg./100ml. fructose, 5·9–23·1 mg./100 ml. ergothioneine, and 32–156 mg./100 ml. citric acid.2. The sequence with which the different parts of boar semen follow one another at ejaculation was studied by the method of fractionate collection. The earliest to be emitted was the ‘pre-sperm fraction’ from which sperm and seminal vesicle secretion were absent. The next was the ‘sperm-rich fraction’ which showed not only a high sperm content but contained also substantial quantities of substances derived from the seminal vesicles, i.e. fructose, ergothioneine and citric acid. The ‘post-sperm fraction’ consisted mainly of accessory gland secretions.3. The analysis of semen fractions collected at very brief intervals has shown that whereas the secretion of the seminal vesicles accompanies the spermatozoa at ejaculation, it attains its maximum immediately after the bulk of the sperm has been ejaculated.
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