Abstract
The complementary substances of an active serum were supposed to be extremely labile bodies, but their stability has never been tested chemically. In this study, the action of various acids, alkalies and salts upon complements has been examined. The list of chemicals used is as follows: ACIDS — hydrochloric, nitric, sulphuric, phosphoric, formic, acetic, propionic, lactic, butyric, oxybutyric, oxalic, tartaric, citric, fumaric, maleinic, citraconic, itaconic, glycerophosphoric, uric and nucleic; ALKALIES — ammonium hydrate, sodium hydrate, magnesium hydrate, calcium hydrate and barium hydrate; SALTS — sodium carbonate; magnesium sulphate, phosphate, acetate and carbonate; calcium sulphate, nitrate, phosphate, acetate, oxalate and carbonate; barium sulphate, phosphate and carbonate. Urea was also included.It was found that all acids and alkalies are able to inactivate complements when used in sufficient concentrations. With monobasic acids it takes about 1 c.c. of n/40 solution to inactivate 1 c.c. of acti...
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