Abstract
The bacterial leaf spot of alfalfa caused by Xanthomonas phaseoli f. sp. alfalfae was observed for the first time in Japan at the farm of National Institute of Animal Industry, in September 1962.Leaf spots are at first minute, round, watersoaked. Mature spots 2-3mm in diameter are pale yellow with a dark brown margin surrounded by a yellowish halo. The spots tend to coalesce along midrib and near the tip or the margin of the leaflets. Severely infected leaves often shed.The causal bacteria infected Phaseolus vulgaris by inoculation producing symptom similar to that produced by Xanthomonas phaseoli on the same host.The morphological, cultural and phsiological characters of the bacteria causing the disease were investigated, and hardly distinguishable from those described by Riker et al. (1935) and Sabet (1959).Therefore the causal bacteria were quite identical in all its characteristics with Xanthomonas phaseoli f. sp. alfalfae (Riker, Jones et Davis) Sabet.
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