Abstract
1. 1. In an extension of previous studies which demonstrated the existence of an association of glycolytic components in extracts of rat skeletal muscle, the properties of this complex have been further investigated. 2. 2. It has been shown that this association of glycolytic enzymes may also be observed in extracts of ovine muscle under physiological conditions of pH and ionic strength, and that the association is sensitive to variations of pH and the concentrations of proteins and specific metabolites such as ATP and fructose 1,6-diphosphate. Furthermore, myosin was identified as a necessary component for complex formation under the experimental conditions. 3. 3. The size of the observed complex of glycolytic enzymes increases with increasing protein concentration; at 52 mg protein/ml, s 20,w values of over 100 are indicated. 4. 4. These data have been discussed in relation to the possible physiological significance of the multi-enzyme association.
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