Abstract
The dependence of optical density upon extinction coefficient is examined for light absorption measurements in which the number of absorbing molecules encountered by each ray of the measuring light is not constant. Some general results applicable to all such situations are derived. Particular attention is given to measurements of the absorption spectra of molecules produced by transverse irradiation of rigid solutions (triplet-triplet spectra). The conditions are determined under which such absorption is most intense, and the deviation from proportionality between optical density and extinction coefficient is least.
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