On-site sample preparation of trace aromatic amines in environmental waters with monolith-based multichannel in-tip microextraction apparatus followed by HPLC determination

Abstract

A novel on-site preparation strategy for the determination of trace aromatic amines (AAs) in environmental waters was developed in the present study. To extract AAs effectively, 4-vinylbenzoic acid was copolymerized with ethylene dimethacrylate (ED)/divinylbenzene (DVB) in a pipette tip to obtain a new monolith-based adsorbent (MBA). The MBAs were employed as the extraction phases of home-made multichannel in-tip microextraction apparatus (ITMA) which was used to perform field sample preparation of AAs in different water samples followed by HPLC/DAD analysis. Due to the abundant functional groups, the prepared MBA displayed satisfying extraction performance for studied AAs. Under the optimized conditions, limits of detection varied from 2.1 to 26 ng/L with good coefficients of determination and precision. The recoveries for real water samples with different fortified concentrations were in the range of 78.1–119%, and the RSD values varied from 0.85 to 11%. In addition, the results achieved with the introduced method were well comparable to that obtained with conventional laboratory sample pretreatment process. Compared with existing approaches, the proposed method exhibits some merits such as s high throughput, good sensitivity and eco-friendliness. Most of important, the MBA/ITMA for on-site preparation avoids the storage and transportation of large volumes of waters, and guarantees the analytical accuracy of studied AAs.