On-site extraction of phenoxycarboxylic acid herbicides in environmental waters utilizing monolith-based in-tip microextraction technique

Abstract

On-site extraction plays a significant role in the reliable quantification of strong polar phenoxycarboxylic acid herbicides (PCAs) in aqueous samples. In current study, a new technique for the field sample preparation of PCAs was developed by means of three channels in-tip microextraction device (TCIM). To capture PCAs effectively, an extraction phase based on monolith (EPM) using vinylimidazole and divinylbenzene/ethylene dimethacrylate as monomer and cross-linkers, respectively, was in-situ synthesized in pipette tips. The EPM fabricated at optimal conditions were characterized by a series of techniques and employed as the adsorbent of TCIM for the on-site extraction of PCAs. The adsorption isotherm was studied so as to inspect the extraction behaviors of EPM towards PCAs. Results revealed that the proposed EPM/TCIM presented satisfactory extraction performance towards PCAs through multiple interactions. The enrichment factors and adsorption capacity were 74-277 and 20 mg/g, respectively. Under the most beneficial extraction parameters, the developed EPM/TCIM was successfully employed to on-site extract PCAs, and then combining with HPLC equipped with diode array detector to monitor trace PCAs in actual waters. The limits of detection (LODs) towards investigated PCAs varied from 0.071 μg/L to 0.30 μg/L. In addition, the accuracy of established approach was inspected with documented method. Compared with existing lab-based sample preparation approaches, the introduced field sample preparation technique exhibits some merits such as avoidance of transporting large volume of water, prevention of analytes loss during sampling procedure, less usage of organic solvent and achievement of satisfactory efficient in sample preparation.