On Site Application of Pseudomonas Aeruginosa ETL-1942 and Bacillus Cereus ETL-1949 in Decolorization and Degradation of Remazol Black-B

Abstract

In the present study an attempt was made to examine the potential of two bacterial strains for decolorization of Remazol Black-B. The strain, isolated from textile effluent treatment plant was characterized on the basis of morphological, biochemical & genotypic characteristics & it was identified as Pseudomonas aeruginosa & Bacillus cereus. The effect of pH, temperature and initial concentration of dye was studied with an aim to determine the optimal conditions. The bacterial strains used in the study were Pseudomonas aeruginosa. ETL-1942 & Bacillus cereus ETL-1949. Out of this Pseudomonas aeruginosa. ETL-1942 emerged out to be most potent decolorizer, being selected for further studies. The selected bacterium shows higher decolorization in static condition as compared to shaking condition. The optimum pH was 7.0. It shows good decolorization efficiency even in alkaline region. The optimum temperature was 37C. The strain could decolorize Remazol Black-B (250 mg/l) by 94% within 24 h under static condition, pH 7.0, temperature of 37C and initial dye concentration of 250 mg/l. Biodegradation and decolorization was confirmed using UV-VIS spectrophotometry, thin layer chromatography (TLC) and fourier transform infrared spectroscopy (FTIR) analysis. The study confirmed the potential of Pseudomonas aeruginosa ETL-1942 in the bioremediation of Remazol Black-B.