On-line Thermal Desorption–Gas Chromatography of Intact Insects for Pheromone Analysis

Abstract

By using a thermodesorption system (TDS) together with a programmable temperature vaporizer (PTV) injector, we confirmed the composition of the sex pheromone of Adoxophyes orana (Lepidoptera: Tortricidae) and Campylomma verbasci (Heteroptera: Miridae) from a single insect per analysis. Intact females and males or pheromone glands were placed in the oven part of the TDS, which was subsequently heated. The compounds released were transferred to the PTV, which was cooled to −150°C. Injection was on a dual-column GC by heating the PTV rapidly to 250°C. The major sex pheromone compounds of A. orana were found only in the pheromone gland of females. Male and female C. verbasci showed fingerprint-identical chromatograms, except for the two sex pheromone compounds, which were present only in females. No distinct differences were found in compounds released from female and male Lygocoris pabulinus (Heteroptera: Miridae). The advantages of this rapid method are the high sensitivity and the low degree of degradation and contamination. This technique was effective in analyzing small insects by gas chromatography–mass spectrometry (GC-MS) without prior manipulation, such as solvent extraction or distillation.