Abstract
An on-line two-dimensional open tubular column supercritical fluid chromatograph was constructed and evaluated. A rotary valve interface allowed independent flow control of two 50-μm i.d. open tubular columns, providing maximum versatility for heartcutting. A solvent-venting injection technique was incorporated in the system that enabled single or multiple 2.0-μL volumes to be injected into an uncoated, yet deactivated, length of capillary precolumn without flooding of the analytical column. A cold trap was employed to refocussolutes from single or multiple fractional cuts after being transferred to the second dimension. The performance of the system was demonstrated with the analysis of mixtures of polycyclic aromatic compounds and steroids. Efficiencies of 4,500 plates m−1 were preserved in the second column after heartcutting.
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