On-Line Monitoring the Growth of E. coli or HeLa Cells Using an Annular Microelectrode Piezoelectric Biosensor.

Feifei Tong,Yan Lian,Junliang Han

doi:10.3390/ijerph13121254

Abstract

Biological information is obtained from the interaction between the series detection electrode and the organism or the physical field of biological cultures in the non-mass responsive piezoelectric biosensor. Therefore, electric parameter of the electrode will affect the biosensor signal. The electric field distribution of the microelectrode used in this study was simulated using the COMSOL Multiphysics analytical tool. This process showed that the electric field spatial distribution is affected by the width of the electrode finger or the space between the electrodes. In addition, the characteristic response of the piezoelectric sensor constructed serially with an annular microelectrode was tested and applied for the continuous detection of Escherichia coli culture or HeLa cell culture. Results indicated that the piezoelectric biosensor with an annular microelectrode meets the requirements for the real-time detection of E. coli or HeLa cells in culture. Moreover, this kind of piezoelectric biosensor is more sensitive than the sensor with an interdigital microelectrode. Thus, the piezoelectric biosensor acts as an effective analysis tool for acquiring online cell or microbial culture information.

Highlights

In recent years, piezoelectric biosensors [1,2] have been applied in numerous fields, including microbiological culture assays or cell biology research
The electric field spatial distributions of microelectrodes with different finger or gap widths in the medium were separately simulated under static conditions in three-dimensional (3D) space using
AreResults shown in Findings that both thethat absolute the absolute value of the piezoelectric frequency shift and the shift amplitude increased with value of the piezoelectric frequency shift and the shift amplitude increased with increasing number increasing numberannuluses

Summary

Introduction

Piezoelectric biosensors [1,2] have been applied in numerous fields, including microbiological culture assays or cell biology research. The signal module of the sensor is composed of two main parts, namely, the biological information recognition component (receptor) and the signal-converting component (transducer). The receptor is used to identify the physical or chemical information of cultured biological objects. With the development of microelectronic manufacturing technology and microsensors, many array microelectrodes have been used as the piezoelectric biosensor receptor. The interdigital microelectrode (IDME) exhibits more advantages than conventional electrodes, such as a micro-scale structure, a high steady-state current density, a low ohm/voltage drop, and a rapid response time and mass transfer between the electrodes [3,4,5]. An IDME can be used in the electrolyte solution system with a high impedance background to detect cell or microbial culture [6]

Methods

Results

Discussion

Conclusion