Abstract
An on-line microfluidic sensing device with an enzyme-modified pre-cell coupled to an amperometric detector for the monitoring of paracetamol in pharmaceutical formulations is described. Horseradish peroxidase (HRP) [EC 1.11.1.7], immobilized on a 3 μl pre-cell, in presence of hydrogen peroxide catalyses the oxidation of paracetamol to N-acetyl- p-benzoquinoneimine. The electrochemical reduction back to hydroquinone is detected on glassy carbon electrode surface at −0.10 V. The recovery of paracetamol from 10 samples ranged from 99.00 to 101.10%. This method could be used to determine paracetamol concentration in the range 0.35–100 μM ( r = 0.997) with a limit of detection of 3.0 × 10 −7 M and a relative standard deviation was less than 4.1% ( n = 8). The method was successfully applied for the processing of as many as 20 samples per hour of paracetamol in pharmaceutical formulations.
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