Abstract
Lipase-catalyzed kinetic resolution of 3-hydroxyoctanoic acid methylester in supercritical carbon dioxide has been investigated. The reaction progress was monitored on-line following the absorbance of the by-product acetic aldehyde using a UV photometer equipped with a high pressure flow through cell. The conversion determined by this method was in very good accordance with results obtained by off-line sampling and subsequent analysis by gas chromatography using a chiral column.
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