Abstract
Four water‐soluble vitamins, thiamine (B1), riboflavin (B2), pantothenic acid (B5), and pyridoxine (B6), frequently found in multivitamin tablets were analyzed by LC‐FTIR. The samples were run on a C18 column using gradient elution with a mobile phase of 0.01 M ammonium acetate and methanol. The separation was carried out on a LC‐FTIR system with a solvent elimination mode. The interface between high performance liquid chromatography (HPLC) and FTIR, which included an ultrasonic nebulizer and a heated drift tube removed the solvent from the HPLC effluent prior to FTIR detection. The analytes were deposited on a moving zinc selenide plate and FTIR spectra of the solid analytes were obtained. Spectral profiles of the vitamins of interest were constructed from the recorded standard spectra. The vitamins of interest in three multivitamin tablets were separated and identified, based on retention time and infrared spectral matching to those of standards.
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