Abstract
Storage of the effluent from a reversed-phase column liquid chromatography separation on a thin-layer chromatography plate offers two advantages: (1) detection principles that are not applicable on-line with flow systems can be used; (2) the thin-layer chromatography plate itself allows a second separation to be carried out. Both advantages are demonstrated for the separation and identification of polycyclic aromatic hydrocarbons in a marine sediment sample. The effluent from a microbore liquid chromatography column was deposited on a linearly moving thin-layer chromatography plate through a spray jet assembly interface. As with isocratic liquid chromatography, not all polycyclic aromatic hydrocarbons present in the sample could be separated; a second separation of the immobilized chromatogram was applied by means of thin-layer chromatography. The overlapping peaks were then resolved completely. Eleven polycyclic aromatic hydrocarbons were identified by measuring their fluorescence excitation and emission spectra on the thin-layer chromatography plate with a conventional spectrometer.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
