Abstract
For a most complete histochemical detection of acid mucopolysaccharides a following modification of Hell's method is recommended: 1. In order to obtain a colloidal ferric hydroxide, 8–12 mg of 10% solution of ferric chloride should be added in drops to 100 mg of boiling distilled water. 2. Dialyzed colloidal ferric hydroxide is mixed with 2 M acetic acid in proportion 3∶1 or 2∶1. 3. The period of action of ferric acetate and solution of ferricyanide upon the preparation is increased to 20–25 minutes. 4. Material in 1–4% solution of basic lead acetate (or in 0.5% solution of acetic acid) is fixed over 8% formalin.
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