Abstract
The diatom concentration of a uniform lake sediment sample was estimated in 15 laboratories. Whilst 21 of 35 estimates were between 13 and 19 × 107 valves g-1, values ranged from 8.2 × 106 to 2.0 × 109 valves g-1. However, counts from replicate sediment digestions, replicate slide preparations from a single digested slurry, and multiple counts from an individual slide all yielded internally consistent results, with acceptably low coefficients of variation (±4.5–6.3%). Without greater efforts towards harmonization, for example by calibrating techniques to a standard of known concentration, reported diatom concentration values, as well as derived data such as diatom accumulation rates, are not directly comparable between most laboratories. This sharply contrasts the strong reproducibility of diatom relative frequency data. A calibration exercise, using the Eucalyptus pollen spike technique to estimate a known concentration of Lycopodium spores, demonstrates that this method performs best when approximately equal proportions of microfossils and introduced markers are counted.
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