On-Chip Tunable Cell Rotation Using Acoustically Oscillating Asymmetrical Microstructures.

Lin Feng,Yonggang Jiang,Bin Song,Deyuan Zhang,Fumihito Arai

doi:10.3390/mi9110596

Lin Feng, Yonggang Jiang + Show 3 more

Open Access

https://doi.org/10.3390/mi9110596

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Abstract

The precise rotational manipulation of cells and other micrometer-sized biological samples is critical to many applications in biology, medicine, and agriculture. We describe an acoustic-based, on-chip manipulation method that can achieve tunable cell rotation. In an acoustic field formed by the vibration of a piezoelectric transducer, acoustic streaming was generated using a specially designed, oscillating asymmetrical sidewall shape. We also studied the nature of acoustic streaming generation by numerical simulations, and our simulation results matched well with the experimental results. Trapping and rotation of diatom cells and swine oocytes were coupled using oscillating asymmetrical microstructures with different vibration modes. Finally, we investigated the relationship between the driving voltage and the speed of cell rotation, showing that the rotational rate achieved could be as large as approximately 1800 rpm. Using our device, the rotation rate can be effectively tuned on demand for single-cell studies. Our acoustofluidic cell rotation approach is simple, compact, non-contact, and biocompatible, permitting rotation irrespective of the optical, magnetic, or electrical properties of the specimen under investigation.

Highlights

Cell manipulations have attracted great attention in the field of modern bioscience [1,2] because the cell is the basic structural, functional, and biological unit of all known living organisms
Cell rotation is one of the fundamental techniques of cell manipulations, which plays a key role in various fields, including cell observation [3], cell analysis [4,5,6], and drug discovery [7]
Diatom cells can be set in an ordered alignment by specific rotational manipulation, which is utilized to achieve detection in screening, diagnosis, and medicine

Summary

Introduction

Cell manipulations have attracted great attention in the field of modern bioscience [1,2] because the cell is the basic structural, functional, and biological unit of all known living organisms. As of yet, no detailed research has been performed on high-speed rotational manipulation of big-size cells like swine oocytes.

Results

Conclusion