On-cartridge derivatisation using a calixarene solid-phase extraction sorbent for facile, sensitive and fast determination of formaldehyde in beer

Abstract

This work demonstrates the successful application of an on-cartridge derivatisation procedure for facile, fast and sensitive determination of formaldehyde in beer by HPLC-UV. The derivatisation and solid-phase extraction (SPE) were integrated into a novel calixarene SPE sorbent: tetraazacalix[2]arene[2]triazine bonded silica gel. Specifically, 2,4-dinitrophenylhydrazine was adsorbed onto the sorbent in advance, based on the charge-transfer interaction between the macrocyclic molecule and nitrobenzenes. The method was optimised and validated: under the optimal conditions of derivatisation, SPE and HPLC separation, good linearity was obtained in the range of 0.080–3.2μgmL−1 with a correlation coefficient of 0.9939, the limit of detection was 3.0ngmL−1 (S/N=3), the limit of quantification was 10ngmL−1 (S/N=10), and the recovery level using this method was desirable at 75–84%. The developed method was successfully applied to determine formaldehyde content in real beer samples; the results were in the range of 0.11–1.1μgmL−1.