Abstract
During a visit to Kakinada, Andhra Pradesh (India) in April, 1956, the writer examined thirty specimens of the frog Rana cyanophlyctis and found that every one of them was heavily infected with an interesting nematode in the rectum. Large numbers of males and females of this parasite were collected and fixed in 5% formalin containing 10% glycerine. Specimens thus fixed and preserved could easily be dissected for preparing mounts of reproductive organs for detailed study. Before being dissected the preserved worms were at first washed in tapwater and then placed in 70% glycerine alcohol which was allowed to evaporate until the worms were in almost pure glycerine.
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