Abstract
In this article discusses the most commonly used methods of investigation of antioxidants. Six varieties of apples (23-25-64, 23-17-22, 23-20-50, 23-25-51, 23-31-135, Sinap of Samara) were selected as objects of research, from the collection of the Research Institute "Zhigulevsky Gardens" "The harvest of 2016 grown on the territory of the Samara region. For the study was obtained hydroalcoholic extract (1 : 1) at 37 °C for 2 hours. The analysis for the maintenance of the total number of phenolic compounds content of gallic acid, flavonoids catechin content, antioxidant capacity with use of free radical DPPH (2,2-diphenyl-1-pikrilgidrazil), restoring forceb with method of FRAP (ferric reducing antioxidant power), the antioxidant activity in linoleic acid system. A significant number of methods to assess the antioxidant activity of the compounds according to the method systematically recording parameters used to determine antioxidant activity, including quantitative. As a result of a comparative study of the content of phenolic compounds, flavonoids, antiradical ability using the 2,2'-diphenyl-1-picrylhydrazyl reagent method, the FRAP restoring force with 2,4,6-tri(2-pyridyl)-1,3 reagent, 5-triazine, antioxidant activity on the linoleic acid model for six varieties of apples were identified as leaders by individual indicators (phenols - apples of variety 23-20-50, flavonoids - apples of variety 23-20-50, anti-radical ability – apples of variety 23-25-51, FRAP-values – apples of variety 23-20-50, antioxidant ability – Syn up of Samara), and in general for the majority of indicators - apples of variety 23-20-50.
Highlights
Ученые давно уже высказывают мнение, что ухудшение здоровья населения в России напрямую связано с ухудшением качества питания
Determination of antioxidant capacity, total phenolic content and mineral composition of different fruit tissue of five apple cultivars grown in Chile // Chil
The analysis for the maintenance of the total number of phenolic compounds content of gallic acid, flavonoids catechin content, antioxidant capacity with use of free radical DPPH (2,2-diphenyl-1-pikrilgidrazil), restoring forceb with method of FRAP (ferric reducing antioxidant power), the antioxidant activity in linoleic acid system
Summary
Колориметрический метод определения общего содержания фенольных веществ основан на применении реактива Фолина-Чокальтеу. Колориметрию свободных радикалов 2,2-дифенил-1-пикрилгидразила проводили спектрофотометрическим методом на приборе КФК при длине волны 517 нм в кювете толщиной слоя жидкости 10 мм. Определение железовосстанавливающей антиоксидантной способности проводили спектрофотометрическим методом на приборе КФК при длине волны 593 нм в кювете с толщиной слоя жидкости 10 мм. В колбы, снабженные притертой пробкой, к 1.00 мл экстракта концентрацией 0.1 мг/см приливали 1.00 мл 2.51% спиртового раствора линолевой кислоты, 2.00 фосфатного буфера pH 7.0, добавляли 1.00 мл 50% этилового спирта и 1 мл Tween 20. Анализ проводили спектрофотометрическим методом на приборе КФК при длине волны 500 нм в кювете с толщиной слоя жидкости 10 мм.
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