Abstract

OMIP-062: A 14-Color, 16-Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway Samples.

Highlights

  • The immaturity of the immune system in early life renders infants vulnerable to infectious diseases, those of the gastrointestinal and respiratory tracts

  • That techniques are developed that facilitate the characterization of the immune response in small-volume and paucicellular samples, from sites of infection where sampling is limited

  • In designing this OMIP [5] we focused on developing a panel to enumerate the innate lymphoid cell (ILC), granulocyte and T-cell responses in infants, those with a lung infection (Table 1)

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Summary

Introduction

The immaturity of the immune system in early life renders infants vulnerable to infectious diseases, those of the gastrointestinal and respiratory tracts. In designing this OMIP [5] we focused on developing a panel to enumerate the innate lymphoid cell (ILC), granulocyte and T-cell responses in infants, those with a lung infection (Table 1). Airway samples from infants we optimized this panel using a small volume (300 μl) of adult peripheral blood.

Results
Conclusion
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