Abstract
This study investigated the efficacy of Omega-7 isolated from the sea buckthorn oil (Polyvit Co., Ltd, Gangar Holding, Ulaanbaatar, Mongolia) in ovine burn wound healing models. In vitro, proliferation (colony-forming rate) and migration (scratch) assays using cultured primary ovine keratinocytes were performed with or without 0.025% and 0.08% Omega-7, respectively. The colony-forming rate of keratinocytes in the Omega-7 group at 72 and 96 h were significantly higher than in the control (P < 0.05). The percentage of closure in scratch assay in the Omega-7 group was significantly higher than in the control at 17 h (P < 0.05). In vivo, efficacy of 4% Omega-7 isolated from buckthorn oil was assessed at 7 and 14 days in grafted ovine burn and donor site wounds. Telomerase activity, keratinocyte growth factor, and wound nitrotyrosine levels were measured at day 14. Grafted sites: Un-epithelialized raw surface area was significantly lower and blood flow was significantly higher in the Omega-7-treated sites than in control sites at 7 and 14 days (P < 0.05). Telomerase activity and levels of keratinocyte growth factors were significantly higher in the Omega-7-treated sites after 14 days compared to those of control (P < 0.05). The wound 3-nitrotyrosine levels were significantly reduced by Omega-7. Donor sites: the complete epithelialization time was significantly shorter and blood flow at day 7 was significantly higher in the Omega-7-treated sites compared to control sites (P < 0.05). In summary, topical application of Omega-7 accelerates healing of both grafted burn and donor site wounds. Omega-7 should be considered as a cost-efficient and effective supplement therapy for burn wound healing.
Highlights
This study investigated the efficacy of Omega-7 isolated from the sea buckthorn oil (Polyvit Co., Ltd, Gangar Holding, Ulaanbaatar, Mongolia) in ovine burn wound healing models
Optimal bio-physiologic events of the healing process involve the following: appropriate inflammation; angiogenesis; re-epithelialization; mesenchymal cell differentiation, proliferation, and migration; and collagen remodeling—cross-linking and strength[5]. These wound healing events are affected by local factors, such as cytokines, blood cells, extracellular matrix, and parenchymal cells
Telomerase is known to be activated in the epidermis and it plays a significant role in the maintenance of skin cell functions and proliferation, it is not known if telomerase activity is negatively affected by burns[12]
Summary
This study investigated the efficacy of Omega-7 isolated from the sea buckthorn oil (Polyvit Co., Ltd, Gangar Holding, Ulaanbaatar, Mongolia) in ovine burn wound healing models. Telomerase activity and levels of keratinocyte growth factors were significantly higher in the Omega-7-treated sites after 14 days compared to those of control (P < 0.05). Optimal bio-physiologic events of the healing process involve the following: appropriate inflammation; angiogenesis; re-epithelialization; mesenchymal cell differentiation, proliferation, and migration; and collagen remodeling—cross-linking and strength[5]. These wound healing events are affected by local factors, such as cytokines (i.e., keratinocyte growth factor, vascular endothelial growth factor, fibroblast growth factor, transforming growth factor beta, and interleukin-1), blood cells, extracellular matrix, and parenchymal cells. Telomerase is known to be activated in the epidermis and it plays a significant role in the maintenance of skin cell functions and proliferation, it is not known if telomerase activity is negatively affected by burns[12]
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