Omega-7 inhibits inflammation and promotes collagen synthesis through SIRT1 activation

In-Bong Song,Yeon-Kyong Son,Na-Young Lee,Hyejung Gu,Ji-Yun Cha,Jungkee Kwon,Hye-Ju Han

doi:10.1007/s13765-018-0377-1

Abstract

Excessive accumulation of reactive oxygen species (ROS) during oxidative stress accelerates the skin aging process. ROS stimulate inflammatory processes in the skin, leading to activation of matrix metalloprotease-1 (MMP-1). Silent information regulator 1 (SIRT1) controls a broad range of cellular functions including the expression of MMP-1. Omega-7 fatty acids such as palmitoleic acid have many beneficial effects on health, including improvement in cardiovascular risk factors and increased insulin sensitivity. However, the effectiveness of omega-7 fatty acids (herein referred to as omega-7) related to skin aging, characterized by the degradation of collagen and loss of elasticity, remains unclear. We here investigated the effects of palmitoleic acid, a representative omega-7, on collagen regeneration through its ability to activate SIRT1 and inhibit MMP-1 in the presence of hydrogen peroxide (H2O2)-induced oxidative stress in human HaCaT cells. SIRT1 activation by omega-7 decreased signaling levels of nuclear transcription factor kappa B (NF-κB) and inflammatory cytokines. However, inhibition of SIRT1 by sirtinol counteracted the advantage effects of omega-7 in H2O2-treated HaCaT cells. In addition, omega-7 significantly counteracted the decrease in collagen abundance and loss of elasticity induced by H2O2. Consistent with this observation, omega-7 significantly decreased H2O2-induced upregulation of MMP-1 in HaCaT cells. Together, these studies suggest the potential efficacy of SIRT1 in collagen regeneration and indicate that omega-7 is a possible functional food to improve skin health for the prevention of aging.

Highlights

Oxidative stress is considered as a main factor that cause toxic effects through the generation of free radicals and can cause disruptions in normal cell function [1]
We evaluated the ability of omega-7 to regulate expression of Silent information regulator 1 (SIRT1) in HaCaT cells and found that omega-7 increased SIRT1 expression in dose-dependent manner (Fig. 1B)
We showed that omega-7 inhibits inflammatory factors and rescues collagen production through activation of SIRT1 and subsequent downregulation of NFjB and matrix metalloprotease-1 (MMP-1) in H2O2-treated HaCaT cells

Summary

Introduction

Oxidative stress is considered as a main factor that cause toxic effects through the generation of free radicals and can cause disruptions in normal cell function [1]. Oxidative stress is generated by various external stimuli such as excessive exposure to UV, environmental toxins, and heat [2] and leads to the production of reactive oxygen species (ROS) [3]. ROS products can accelerate skin aging by decreasing collagen regeneration and elasticity while simultaneously increasing the abundance of inflammatory factors [4]. Collagen regulation factors such as metalloproteinase-1 (MMP-1) and pro-collagen type 1 (PCOL1) have important roles in oxidative stress generated in the skin [4]. SIRT1 regulates inflammatory responses through deacetylation of nuclear transcription factor kappa B (NF-jB), a major

Methods

Results

Conclusion